validation of a β-me elisa for detection of anti leishmania donovani antibodies in eastern sudan
نویسندگان
چکیده
background: a β-mercaptoethnol (β-me)-treated promastigote antigen of l. donovani was successfully employed in direct agglutination test (dat) for the diagnosis of visceral leishmaniasis (vl). objective: the β-me-treated antigen was further incorporated into an enzyme-linked immunosorbent assay set-up (β-me elisa) and evaluated for vl diagnosis against outcome of reference freeze-dried dat (fd-dat) and rk39 strip test (rkt) commercial kits. methods: two-hundred and ninety-two sera from patients with high vl suspicion of whom 105 had confirmed l. donovani infection were tested. results: relatively higher sensitivities of 93.3% (95% ci: 88.4- 98.2) and 92.4% (95% ci: 87.3-97.5) were determined for β-me elisa and fd-dat as compared to 83.8% (95% ci: 76.7-90.8) for rkt. of 73 vl sera that scored maximum absorbance values (>0.81) in β-me elisa, 66 (90.4%) tested at the highest agglutination titres (>1:51200) in fd-dat as did 56 (76.7%) also at comparable reaction intensities (3 + colour intensity) in rkt. compared with fd-dat (94.7%, 95% ci: 91.5-97.9) or rkt (93.0%, 95% ci: 89.3-96.6), lower specificity was estimated for β-me elisa (90.4%, 95% ci: 86.1-94.6). based both on positive and negative microscopy for l. donovani in organ aspirates of all vl suspects enrolled (292), significantly higher correlation (p
منابع مشابه
Validation of a β-ME ELISA for Detection of Anti Leishmania donovani Antibodies in Eastern Sudan
Background: A β-mercaptoethnol (β-ME)-treated promastigote antigen of L. donovani was successfully employed in direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis (VL). Objective: The β-ME-treated antigen was further incorporated into an enzyme-linked immunosorbent assay set-up (β-ME ELISA) and evaluated for VL diagnosis against outcome of reference freeze-dried DAT (FD-...
متن کاملValidation of a β-ME ELISA for detection of anti Leishmania donovani antibodies in Eastern Sudan.
BACKGROUND A β-mercaptoethnol (β-ME)-treated promastigote antigen of L. donovani was successfully employed in direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis (VL). OBJECTIVE The β-ME-treated antigen was further incorporated into an enzyme-linked immunosorbent assay set-up (β-ME ELISA) and evaluated for VL diagnosis against outcome of reference freeze-dried DAT (FD-...
متن کاملQualitative and Semi-Quantitative Comparison of an Rk39 Strip Test and Direct Agglutination Test for Detection of Anti-Leishmania Donovani Antibodies in the Sudan
Background: Until now, the comparison of the rK39 strip test (RKT) and direct ag-glutination test (DAT) for detection of visceral leishmaniasis (VL) is exclusively based on either positive or negative qualification of the reaction outcome. Objective: In this study, we compared the diagnostic performance of RKT and DAT for VL both qualitatively and semi-quantitatively. Methods: For comparison ba...
متن کاملDetection of antibodies to Leishmania donovani in animals in a kala-azar endemic region in eastern Sudan: a preliminary report.
The prevalence of antibodies against Leishmania donovani in selected domestic and wild animal species in 2 villages in Sudan with active L. donovani transmission in humans was investigated. Screening of domestic animals (donkeys, cows, sheep, goats, camels and dogs) with the direct agglutination test (DAT) detected reaction rates above the cut-off titres in donkeys (68.7%), cows (21.4%) and goa...
متن کاملqualitative and semi-quantitative comparison of an rk39 strip test and direct agglutination test for detection of anti-leishmania donovani antibodies in the sudan
background: until now, the comparison of the rk39 strip test (rkt) and direct ag-glutination test (dat) for detection of visceral leishmaniasis (vl) is exclusively based on either positive or negative qualification of the reaction outcome. objective: in this study, we compared the diagnostic performance of rkt and dat for vl both qualitatively and semi-quantitatively. methods: for comparison ba...
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عنوان ژورنال:
iranian journal of immunologyجلد ۸، شماره ۳، صفحات ۱۵۰-۱۵۸
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